Fig. 4. SUPT5H depletion inhibits the migratory and invasive behavior of MDA-MB-231 cells. (A) A wound-healing assay was performed to measure the migration potential of siRNA-scr and siRNA-SUPT5H transfected cells. The wound area was quantified by ImageJ 64 software. Scale bar, 100µm, and magnification, 10x. (B) Transwell assay was used to assess the migration and invasion potentials of MDA-MB-231 after transfection with siRNA-scr or siRNA-SUPT5H. Scale bar, 100µm, and magnification, 20x. (A, B) data is the representation of three independent experiments. **p<0.01, ***p<0.001. (C) Western blot analysis of the expression of migration-related proteins of MDA-MB-231 cells following transfection of siRNA-scr and siRNA-SUPT5H (representative blots are shown from two independent experiments).